Karan Wangpaiboon, Ph.D.
B.Sc., Chulalongkorn University
Ph.D., Chulalongkorn University
Office: Room 721/1, Science 10 Building
Phone: 662-218-7758
Fax: 662-218-5418
Email: Karan.Wa@chula.ac.th
Research
Carbohydrates are an essential biomolecule for life. They play a broad range of important biological roles including cell structure, cell communication, and energy source, etc. Importantly, Thailand is located in tropical zone, where plants can be grown abundantly and a variety of natural carbohydrate products can be harvested throughout the year. Currently, our research group mainly focuses on carbohydrate-active enzymes in bacteria (e.g., enzymes participating in bacterial biofilm formation) and in plants (i.e., starch-metabolising enzymes). These enzymes are molecular machines that work on their substrates precisely and efficiently. Carbohydrate-active enzymes is such an essential tool for manipulating and modifying carbohydrate structures due to non-template coding with high structural diversities characteristics of carbohydrate which is complicated to be only chemically employed. In addition, understanding of the carbohydrate enzyme mechanisms and the biological roles of their products can lead us to an appropriately cutting-edge application in biotechnology. We believe that the great technologies are always hidden in the nature because they have been evolutionarily invented and selected for huge generations.
Research Topics:
• Structures and mechanisms of carbohydrate-active enzymes
• Carbohydrate metabolism in bacteria and plants
• Formation of sucrose-dependent biofilm in lactic acid bacteria
• Applications of enzymes and carbohydrates in biotechnology
Recent Publications:
2023 Charoenwongphaibun, C., Lorthongpanich, C., Septham, P., Wangpaiboon, K., Panpetch, P., Pichyangkura, R., … & Kuttiyawong, K. (2023). Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery. Gels, 9(3), 188.
2023 Wangpaiboon, K., Panpetch, P., Charoenwongpaiboon, T., Klaewkla, M., & Field, R. A. Cassava pullulanase and its synergistic debranching action with isoamylase 3 in starch catabolism. Frontiers in Plant Science, 14, 138.
2023 Charoenwongpaiboon, T., Wangpaiboon, K., Field, R. A., Prousoontorn, M., & Pichyangkura, R. (2023). Cross-linked enzyme aggregates (combi-CLEAs) derived from levansucrase and variant inulosucrase are highly efficient catalysts for the synthesis of levan-type fructooligosaccharides. Molecular Catalysis, 535, 112827.
2022 Charoenwongpaiboon, T., Wangpaiboon, K., Septham, P., Jiamvoraphong, N., Issaragrisil, S., Pichyangkura, R., & Lorthongpanich, C. (2022). Production and bioactivities of nanoparticulated and ultrasonic-degraded levan generated by Erwinia tasmaniensis levansucrase in human osteosarcoma cells. International Journal of Biological Macromolecules, In Press
2022 Charoenwongpaiboon, T., Wangpaiboon, K., Klaewkla, M., Kuttiyawong, K., Field, R. A., & Pichyangkura, R. (2022). Levan-type fructooligosaccharides synthesis by novel levansucrase-inulosucrase fusion enzyme. Biochemical Engineering Journal, 108524.
2022 Krusong, K., Ismail, A., Wangpaiboon, K., & Pongsawasdi, P. (2022). Production of Large-Ring Cyclodextrins by Amylomaltases (Review article). Molecules, 27(4), 1446.
2021 Wangpaiboon, K., Klaewkla, M., Charoenwongpaiboon, T., Vongkusolkit, N., Panpetch, P., Kuttiyawong, K., … & Pichyangkura, R. (2022). Synergistic enzyme cocktail between levansucrase and inulosucrase for superb levan-type fructooligosaccharide synthesis. Enzyme and Microbial Technology, 154, 109960.
2021 Leerawatthanakun, S., Charoenwongpaiboon, T., Klaewkla, M., Chunsrivirot, S., Sirirak, J., Sriwitool, T. E., Wangpaiboon, K., & Pichyangkura, R. (2021). High surfactant-tolerant β-mannanase isolated from Dynastes hercules larvae excrement, and identification of its hotspot using site-directed mutagenesis and molecular dynamics simulation. Enzyme and Microbial Technology, 145, 109956.
2021 Suksiri, P., Ismail, A., Sirirattanachatchawan, C., Wangpaiboon, K., Muangsin, N., Tananuwong, K., & Krusong, K. (2021). Enhancement of large ring cyclodextrin production using pretreated starch by glycogen debranching enzyme from Corynebacterium glutamicum. International Journal of Biological Macromolecules, 193, 81-87.
2021 Wangpaiboon, K., Laohawuttichai, P., Kim, S. Y., Mori, T., Nakapong, S., Pichyangkura, R., … & Krusong, K. (2021). A GH13 α-glucosidase from Weissella cibaria uncommonly acts on short-chain maltooligosaccharides. Acta Crystallographica Section D: Structural Biology, 77(8), 1064-1076.
2021 Wangpaiboon, K., Sitthiyotha, T., Chunsrivirot, S., Charoenwongpaiboon, T., & Pichyangkura, R. (2021). Unravelling Regioselectivity of Leuconostoc citreum ABK-1 Alternansucrase by Acceptor Site Engineering. International Journal of Molecular Sciences, 22(6), 3229.
2021 Charoenwongpaiboon, T., Wangpaiboon, K., Pichyangkura, R., Nepogodiev, S. A., Wonganan, P., Mahalapbutr, P., & Field, R. A. (2021). Characterization of a nanoparticulate exopolysaccharide from Leuconostoc holzapfelii KM01 and its potential application in drug encapsulation. International Journal of Biological Macromolecules, 187, 690-698.
2021 Charoenwongpaiboon, T., Wangpaiboon, K., & Pichyangkura, R. (2021). Cross-linked levansucrase aggregates for fructooligosaccharide synthesis in fruit juices. LWT, 150, 112080.
2021 Sangpong, L., Khaksar, G., Pinsorn, P., Oikawa, A., Sasaki, R., Erban, A., Watanabe, M., Wangpaiboon, K. … & Sirikantaramas, S. (2021). Assessing dynamic changes of taste-related primary metabolism during ripening of durian pulp using metabolomic and transcriptomic analyses. Frontiers in Plant Science, 12, 1198.
2020 Wangpaiboon, K., Waiyaseesang, N., Panpetch, P., Charoenwongpaiboon, T., Nepogodiev, S. A., Ekgasit, S., … & Pichayangkura, R. (2020). Characterisation of insoluble α-1, 3-/α-1, 6 mixed linkage glucan produced in addition to soluble α-1, 6-linked dextran by glucansucrase (DEX-N) from Leuconostoc citreum ABK-1. International Journal of Biological Macromolecules. 152, 473-482.
2020 Charoenwongpaiboon, T., Punnatin, P., Klaewkla, M., Pramoj Na Ayutthaya, P., Wangpaiboon, K., Chunsrivirot, S., … & Pichyangkura, R. (2020). Conserved Calcium-Binding Residues at the Ca-I Site Involved in Fructooligosaccharide Synthesis by Lactobacillus reuteri 121 Inulosucrase. ACS Omega.
2020 Klaewkla, M., Pichyangkura, R., Charoenwongpaiboon, T., Wangpaiboon, K., & Chunsrivirot, S. (2020). Computational design of oligosaccharide producing levansucrase from Bacillus licheniformis RN-01 to improve its thermostability for production of levan-type fructooligosaccharides from sucrose. International Journal of Biological Macromolecules.
2020 Phengnoi, P., Charoenwongpaiboon, T., Wangpaiboon, K., Klaewkla, M., Nakapong, S., Visessanguan, W., … & Kuttiyawong, K. (2020). Levansucrase from Bacillus amyloliquefaciens KK9 and Its Y237S Variant Producing the High Bioactive Levan-Type Fructooligosaccharides. Biomolecules, 10(5), 692.
2019 Wangpaiboon, K., Pitakchatwong, C., Panpetch, P., Charoenwongpaiboon, T., Field, R. A., & Pichyangkura, R. (2019). Modified properties of alternan polymers arising from deletion of SH3-like motifs in Leuconostoc citreum ABK-1 alternansucrase. Carbohydrate polymers, 220, 103-109.
2019 Nopvichai, C., Pongkorpsakol, P., Wongkrasant, P., Wangpaiboon, K., Charoenwongpaiboon, T., Ito, K., … & Pichyangkura, R. (2019). Galactomannan Pentasaccharide Produced from Copra Meal Enhances Tight Junction Integration of Epithelial Tissue through Activation of AMPK. Biomedicines, 7(4), 81.
2019 Kanjanatanin, P., Pichyangkura, R., Sitthiyotha, T., Charoenwongpaiboon, T., Wangpaiboon, K., & Chunsrivirot, S. (2019). Computational design of Bacillus licheniformis RN-01 levansucrase for control of the chain length of levan-type fructooligosaccharides. International journal of biological macromolecules.
2019 Charoenwongpaiboon, T., Wangpaiboon, K., Panpetch, P., Field, R. A., Barclay, J. E., Pichyangkura, R., & Kuttiyawong, K. (2019). Temperature-dependent inulin nanoparticles synthesized by Lactobacillus reuteri 121 inulosucrase and complex formation with flavonoids. Carbohydrate Polymers, 115044.
2019 Charoenwongpaiboon, T., Klaewkla, M., Chunsrivirot, S., Wangpaiboon, K., Pichyangkura, R., Field, R. A., & Prousoontorn, M. H. (2019). Rational re-design of Lactobacillus reuteri 121 inulosucrase for product chain length control. RSC Advances, 9(26), 14957-14965.
2019 Charoenwongpaiboon, T., Sitthiyotha, T., Ayutthaya, P. P. N., Wangpaiboon, K., Chunsrivirot, S., Prousoontorn, M. H., & Pichyangkura, R. (2019). Modulation of fructooligosaccharide chain length and insight into the product binding motif of Lactobacillus reuteri 121 inulosucrase. Carbohydrate polymers, 209, 111-121.
2019 Charoenwongpaiboon, T., Supraditaporn, K., Klaimon, P., Wangpaiboon, K., Pichyangkura, R., Issaragrisil, S., & Lorthongpanich, C. (2019). Effect of alternan versus chitosan on the biological properties of human mesenchymal stem cells. RSC advances, 9(8), 4370-4379.
2018 Wangpaiboon K., Padungros P., Nakapong S., Charoenwongpaiboon T., Rejzek M., Field R.A., and Pichyangkura R.. (2018) An α-1,6-and α-1,3-linked glucan produced by Leuconostoc citreum ABK-1 alternansucrase with nanoparticle and film-forming properties. Scientific Reports 8:8340, DOI:10.1038/s41598-018-26721-w.
2018 Charoenwongpaiboon T., Wangpaiboon K., Pichyangkura R., and Manchumas Hengsakul Prousoontorn (2018) Highly porous core-shell chitosan beads with superb immobilization efficiency for Lactobacillus reuteri 121 inulosucrase and production of inulin-type fructooligosaccharides. RSC Advances 8:17008–17016, DOI: 10.1039/c8ra02241k.