TH
EN
 
 
Molecular Biology and Genomics of Shrimp

Anchalee Tassankajon, Professor, Ph.D.
Department Head

B.Sc., Chulalongkorn University
M.Sc., University of Montana
Ph.D., University of Montana

Office: Room 723, Science 10 Building
Phone: 662-218-5439
Fax: 662-218-5418
Email: anchalee.k@chula.ac.th

Research

Gene technology has been applied to the cultivation of black tiger shrimp, Penaeus monodon for genetic improvement and disease control. Three research areas have been initiated. The first topic involves the establishment of a well-characterize shrimp EST resource and construction of high quality gene index database for gene finding, gene characterization and EST data mining.

The second topic involves the identification and characterization of bio-defense molecules or immune effectors from the black tiger shrimp. Knowledge in shrimp immunity is of great importance in establishing strategies for disease control. However, little attention has been paid in shrimp immunity. In this study, we aim to identify molecules involved in defense mechanisms in P. monodon by cloning and characterization of their encoding genes and proteins. The expression of the defense genes during the response to infections will be analyzed which will lead to better understanding of their involvement in defense mechanism.

The third topic involves the development of molecular markers e.g. microsatellite , AFLP and SNP markers. Powerful genetic markers are use to evaluate the degree of genetic diversity in natural populations for management of wild stock and to initiate selection for long term management of potential stocks. Genetic markers also have numerous applications in shrimp breeding programmes including identification of natural stocks, family-specific markers and mapping loci for important traits.

Research Topics:

•  Genomic Researches for Increasing Culture Efficiency of the Black Tiger Shrimp ( Penaeus monodon ) Phase II : Large-scale cDNA sequencing, database analysis and cDNA microarray fabrication

•  Antimicrobial immune effector in marine invertebrates: characterization and application for disease control

•  Construction of a genetic linkage map of the black tiger shrimp ( Penaeus monodon ) using AFLP, microsatellite and SNP markers

Research Grants:

2012-2017 Japan International Cooperation Agency (Co-Investigator) “Development of aquaculture technology for food security and food safety in the next generation”

2010-2015 Japan Society for the Promotion of Science-National Research Council of Thailand (Co-Investigator) “Development of new biotechnology for aquaculture and risk management of aquaculture products”

2010-2013 National Research Council of Thailand (Principal Investigator) “Sustainable Shrimp Aquaculture: Biotechnology for Closed and Super-intensive Culture System”

2010-2011 Chulalongkorn University (Principal Investigator), “Preliminary Research for Sustainable Shrimp Aquaculture: Biotechnologyfor Closed and Super-intensive Culture System”

2007-2012 National Center for genetic Engineering and Biotechnology, National Science and Technology Development Agency (Principal Investigator). “ Genomic Research for Increase Culture Efficiency of Shrimp: The Study of Genes and Proteins Associated with Disease Resistance ”

2007-2010 Commission on Higher Education, Grant for Research Group (CHE-RES-RG), Shrimp Molecular Biology and Genomics Laboratory

2005- 2007 National Center for genetic Engineering and Biotechnology, National Science and Technology Development Agency (Principal Investigator). “Genomic Researches for Increasing Culture Efficiency of the Black Tiger Shrimp (Penaeus monodon) Phase II: Large-scale cDNA sequencing, database analysis and cDNA microarray fabrication”

2004 National Center for genetic Engineering and Biotechnology, National Science and Technology Development Agency (Co-Investigator). “Determination of Genetic Diversity, Effective Population Size and Inbreeding Depression Levels of Domesticated Stock of the Black Tiger Shrimp (Penaeus monodon)”

2003 - 2005 National Center for genetic Engineering and Biotechnology, National Science and Technology Development Agency (Co-Investigator). “ Construction of genetic linkage map of the black tiger shrimp (Penaeus monodon) using AFLP, microsatellites and SNP markers”

2003-2004 National Center for genetic Engineering and Biotechnology, National Science and Technology Development Agency (Principal Investigator). “Genomic Researches for Increasing Culture Efficiency of the Black Tiger Shrimp (Penaeus monodon) Phase I: Large-scale cDNA Sequencing and Database Development”

2003-2007 The Ratchadaphisek Somphot Endowment to Shrimp Molecular Biology and Genomics Laboratory.

2002-2005 European commission (Co-investigator). “Anti-infectious immune effectors in marine invertebrates : characterisation and application for disease control in aquaculture”

2000-2003 National Center for genetic Engineering and Biotechnology, National Science and Technology Development Agency (Principal Investigator). “Identification of defense molecules in the black tiger shrimp, Penaeus monodon : cloning and expression of genes encoding defense molecules from shrimp hemocyte cDNA libraries”.

Publications

  1. Jatuyosporn, T.; Supungul, P.; Tassanakajon, A.Krusong, K.. The essential role of clathrin-mediated endocytosis in yellow head virus propagation in the black tiger shrimp Penaeus monodon.   Developmental and Comparative Immunology 2014, 44 (1), 100-110.
  2. Yingvilasprasert, W.; Supungul, P.; Tassanakajon, A.. PmTBC1D20, a Rab GTPase-activating protein from the black tiger shrimp, Penaeus monodon, is involved in white spot syndrome virus infection. Developmental and Comparative Immunology 2014, 42 (2), 302-310.
  3. Sookruksawong, S.; Sun, F.; Liu, Z.; Tassanakajon, A. RNA-Seq analysis reveals genes associated with resistance to Taura syndrome virus (TSV) in the Pacific white shrimp Litopenaeus vannamei. Developmental and Comparative. Immunology 2013, 41 (4) , 523-533.
  4. Amparyup, P.; Promrungreang, K.; Charoensapsri, W.; Sutthangkul, J.; Tassanakajon, A. A serine proteinase PmClipSP2 contributes to prophenoloxidase system and plays a protective role in shrimp defense by scavenging lipopolysaccharide. Developmental and Comparative Immunology 2013, 41 (4) , 597-607.
  5. Wetsaphan, N., Rimphanitchayakit, V., Tassanakajon, A., Somboonwiwat, K. PmSERPIN3 from black tiger shrimp Penaeus monodon is capable of controlling the proPO system, Developmental and Comparative Immunology 2013, 41 (2), 110-119.
  6. Sookruksawong, S.; Pongsomboon, S.; Tassanakajon, A. Genomic organization of the cytosolic manganese superoxide dismutase gene from the Pacific white shrimp, Litopenaeus vannamei, and its response to thermal stress. Fish and Shellfish Immunology 2013, Article in Press
  7. Amparyup, P.; Promrungreang, K.; Charoensapsri, W.; Sutthangkul, J.; Tassanakajon, A. A novel clip domain serine proteinase PmClipSP2 functions in shrimp prophenoloxidase system and acts as a scavenger protein for lipopolysaccharide. Fish & Shellfish Immunology 2013, 34(6), 1637-1637.
  8. Charoensapsri, W.; Sutthangkul, J.; Amparyup, P.; Senapin, S.; Tassanakajon, A. Melanization cascade of shrimp and its importance for white spot syndrome virus infection. Fish & Shellfish Immunology 2013, 34(6), 1645-1646.
  9. Ponprateep, S.; Phiwsaiya, K.; Tassanakajon, A.; Rimphanitchayakit, V. Interaction between Kazal serine proteinase inhibitor SPIPm2 and viral protein WSV477 reduces the replication of white spot syndrome virus. Fish and Shellfish Immunology 2013, 35 (3) , 957-964.
  10. Somboonwiwat, K.; Wetsaphan, N.; Mingmongkol, S.; Jaree, P.; Tharntada, S.; Tassanakajon, A.; Rimphanitchayakit, V. Penaeus monodon serpins inhibit prophenoloxidase activation. Fish & Shellfish Immunology 2013, 34(6 ), 1678-1678.
  11. Tassanakajon, A. Innate immune system of shrimp. Fish and Shellfish Immunology 2013, 34(4), 953.
  12. Amparyup, P.; Charoensapsri, W.; Tassanakajon, A. Prophenoloxidase system and its role in shrimp immune responses against major pathogens. Fish and Shellfish Immunology 2013, 34(4), 990-1001.
  13. Tassanakajon, A.; Somboonwiwat, K.; Supungul, P.; Tang, S. Discovery of immune molecules and their crucial functions in shrimp immunity. Fish and Shellfish Immunology 2013, 34(4), 954-967.
  14. Wu C., Charoensapsri W., Nakamura S., Tassanakajon A., Soderhall I., Soderhall K. An MBL-like protein may interfere with the activation of the proPO-system, an important innate immune reaction in invertebrates. Immunobiology 2013, 218(2), 159–168.
  15. Rattanachai, A.; Supungul, P.; Srisapoome, P.;Poompuang, S.; Tassanakajon, A. Sequence Information, Ontogeny and Expression Analysis of Complement Component C3 in Walking Catfish Clarias macrocephalus. Thai Journal of Veterinary Memicine 2012, 42(4), 495-507.
  16. Jaree, P.; Tassanakajon, A.; Somboonwiwat, K. Effect of the anti-lipopolysaccharide factor isoform 3 (ALFPm3) from Penaeus monodon on Vibrio harveyi cells. Developmental and Comparative Immunology 2012, 38(4), 554-560.
  17. Chaikeeratisak V., Somboonwiwat K., Tassanakajon A., Shrimp Alpha-2-Macroglobulin Prevents the Bacterial Escape by Inhibiting Fibrinolysis of Blood Clots. journal.pone 2012, 7(10), 1-10.
  18. Suthianthong, P., Donpudsa, S., Supungul, P., Tassanakajon A., Rimphanitchayakit V., The N-terminal glycine-rich and cysteine-rich regions are essential for antimicrobial activity of crustinPm1 from the black tiger shrimp Penaeus monodon. Fish & Shellfish Immunology 2012, 33, 977-983.
  19. Somnuk S.; Tassanakajon A.; Rimphanitchayakit V.(2012), Gene expression and characterization of a serine proteinase inhibitor PmSERPIN8 from the black tiger shrimp Penaeus monodon, Fish & Shellfish Immunology 33, 332-341.
  20. Chaikeeratisak V.; Somboonwiwat K.; Wang H.C.; Lo C.F.; Tassanakajon A. Proteomic analysis of differentially expressed proteins in the lymphoid organ of Vibrio harveyi-infected Penaeus monodon. Mol. Biol. Rep. 2012, 39(5), 6367-6377.
  21. Amparyup P.; Sutthangkul J.; Charoensapsri W. ; Tassanakajon A., Pattern Recognition Protein Binds to Lipopolysaccharide and beta-1,3-Glucan and Activates Shrimp Prophenoloxidase System. J. Biol. Chem. 2012, 287(13), 10060-10069.
  22. Vatanavicharn T., Pongsomboon S., Tassanakajon A., Two plasmolipins from the black tiger shrimp, Penaeus monodon and their response to virus pathogens.Developmental and Comparative Immunology 2012, 38, 389–394.
  23. Ponprateep, Sirikwan; Tharntada, Sirinit; Somboonwiwat, Kunlaya; et al. Gene silencing reveals a crucial role for anti-lipopolysaccharide factors from Penaeus monodon in the protection against microbial infections Source: FISH & SHELLFISH IMMUNOLOGY 2012, 32, 26-34
  24. Krusong, K.; Poolpipat, P.; Supungul, P.; Tassanakajon, A. (2012), A comparative study of antimicrobial properties of crustinPm1 and crustinPm7 from the black tiger shrimp Penaeus monodon. Developmental and Comparative Immunology, 36, (1), 208-215.
  25. Ponprateep, S.; Tassanakajon, A.; Rimphanitchayakit, V. (2011), A Kazal type serine proteinase SPIPm2 from the black tiger shrimp Penaeus monodon is capable of neutralization and protection of hemocytes from the white spot syndrome virus. FISH & SHELLFISH IMMUNOLOGY Volume: 31 Issue: 6 Pages: 1179-1185.
  26. Kaeodee, M., Pongsomboon, S., Tassanakajon, A. (2011). Expression analysis and response of Penaeus monodon 14-3-3 genes to salinity stress. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 159: 244-251.
  27. Suthianthong, P., Pulsook, N., Supungul, P., Tassanakajon, A., Rimphanitchayakit, V. (2011). A double WAP domain-containing protein PmDWD from the black tiger shrimp Penaeus monodon is involved in the controlling of proteinase activities in lymphoid organ. Fish and Shellfish Immunology. 30: 783-790.
  28. Imjongjirak, C., Amparyup, P., Tassanakajon, A. (2011). Two novel antimicrobial peptides, arasin-likeSp and GRPSp, from the mud crab Scylla paramamosain, exhibit the activity against some crustacean pathogenic bacteria. Fish and Shellfish Immunology. 30: 706-712.
  29. Woramongkolchai, N., Supungul, P., Tassanakajon, A. (2011). The possible role of penaeidin5 from the black tiger shrimp, Penaeus monodon, in protection against viral infection. Developmental and Comparative Immunology. 35: 530-536.
  30. Imjongjirak, C., Amparyup, P., Tassanakajon, A. (2011). Molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (ALF) from the mud crab, Scylla paramamosain. Fish and Shellfish Immunology. 30: 58-66.
  31. Charoensapsri, W., Amparyup, P., Hirono, I., Aoki, T., Tassanakajon, A. (2011). PmPPAE2, a new class of crustacean prophenoloxidase (proPO)-activating enzyme and its role in PO activation. Developmental and Comparative Immunology. 35: 115-24.
  32. Pongsomboon, S., Tang, S., Boonda, S., Aoki, T., Hirono, I., Tassanakajon, A. (2011). A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens. Fish and Shellfish Immunology. 30: 439-46
  33. Imjongjirak, C., Amparyup, P., Tassanakajon, A. (2010). Molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (ALF) from the mud crab, Scylla paramamosain. Fish and Shellfish Immunology. 30: 58-66  
  34. Charoensapsri, W., Amparyup, P., Hirono, I., Aoki, T., Tassanakajon, A. (2011). PmPPAE2, a new class of crustacean prophenoloxidase (proPO)-activating enzyme and its role in PO activation. Developmental and Comparative Immunology. 35: 115-124.
  35. Donpudsa, S., Ponprateep, S., Prapavorarat, A., Visetnan, S., Tassanakajon, A., Rimphanitchayakit, V. (2010). A Kazal-type serine proteinase inhibitor SPIPm2 from the black tiger shrimp Penaeus monodon is involved in antiviral responses. Developmental and Comparative Immunology. 34: 1101-1108.
  36. Donpudsa, S., Rimphanitchayakit, V., Tassanakajon, A., Soderhall, I., Soderhall, K. (2010). Characterization of two crustin antimicrobial peptides from the freshwater crayfish Pacifastacus leniusculus. Journal of Invertebrate Pathology. 104: 234-238.
  37. Donpudsa, S., Soderhall, I., Rimphanitchayakit, V., Cerenius, L., Tassanakajon, A., Soderhall, K. (2010). Proteinase inhibitory activities of two two-domain Kazal proteinase inhibitors from the freshwater crayfish Pacifastacus leniusculus and the importance of the P2 position in proteinase inhibitory activity. Fish and Shellfish Immunology. 29: 716-723.
  38. Homvises, T., Tassanakajon, A., Somboonwiwat, K. (2010). Penaeus monodon SERPIN, PmSERPIN6, is implicated in the shrimp innate immunity. Fish and Shellfish Immunology. 29: 890-898.
  39. Somboonwiwat, K., Chaikeeratisak, V., Wang, H.C., Fang Lo, C., Tassanakajon, A. (2010). Proteomic analysis of differentially expressed proteins in Penaeus monodon hemocytes after Vibrio harveyi infection. Proteome Science. 8.
  40. Tassanakajon, A., Amparyup, P., Somboonwiwat, K., Supungul, P. (2010). Cationic Antimicrobial Peptides in Penaeid Shrimp. Marine Biotechnology. 12: 487-505. Review Article
  41. Prapavorarat, A., Vatanavicharn, T., Soderhall, K., Tassanakajon, A. (2010). A novel viral responsive protein is involved in hemocyte homeostasis in the black tiger shrimp, Penaeus monodon. Journal of Biological Chemistry 285: 21467-21477
  42. Proespraiwong, P., Tassanakajon, A., Rimphanitchayakit, V. (2010). Chitinases from the black tiger shrimp Penaeus monodon: phylogenetics, expression and activities. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology. 156: 86-96.
  43. Supungul, P., Rimphanitchayakit, V., Aoki, T., Hirono, I., Tassanakajon, A. (2010). Molecular characterization and expression analysis of a C-type and two novel muramidase-deficient I-type lysozymes from Penaeus monodon. Fish and Shellfish Immunology. 128: 490-498.    
  44. Prapavorarat, A., Pongsomboon, S., Tassanakajon, A. (2010). Identification of genes expressed in response to yellow head virus infection in the black tiger shrimp, Penaeus monodon, by suppression subtractive hybridization. Developmental and Comparative Immunology. 34: 611-617.  
  45. Rimphanitchayakit, V., Tassanakajon, A. (2010). Structure and function of invertebrate Kazal-type serine proteinase inhibitors. Developmental and Comparative Immunology 334:377-386. Review Article
  46. Cerenius, L., Liu, H., Zhang, Y., Rimphanitchayakit, V., Tassanakajon, A., Gunnar Andersson, M., Söderhäll, K., Söderhäll, I. (2010). High sequence variability among hemocyte-specific Kazal-type proteinase inhibitors in decapod crustaceans. Developmental and Comparative Immunology. 34: 69-75.
  47. Amparyup, P., Wiriyaukaradecha, K., Charoensapsri, W., Tassanakajon, A. (2010). A clip domain serine proteinase plays a role in antibacterial defense but is not required for prophenoloxidase activation in shrimp.Developmental and Comparative Immunology. 34: 168-176.
  48. Amparyup, P., Charoensapsri, W., Tassanakajon, A. (2009). Two prophenoloxidases are important for the survival of Vibrio harveyi challenged shrimp Penaeus monodon. Developmental and Comparative Immunology. 33: 247-256.
  49. Charoensapsri, W., Amparyup, P., Hirono, I., Aoki, T., Tassanakajon, A. (2009). Gene silencing of a prophenoloxidase activating enzyme in the shrimp, Penaeus monodon, increases susceptibility to Vibrio harveyi infection. Developmental and Comparative Immunology. 33: 811-820.
  50. Donpudsa, S., Tassanakajon, A., Rimphanitchayakit, V. (2009). Domain inhibitory and bacteriostatic activities of the five-domain Kazal-type serine proteinase inhibitor from black tiger shrimp Penaeus monodon. Developmental and Comparative Immunology. 33: 481-488.
  51. Jitvaropas, R., Amparyup, P., Gross, P.S., Tassanakajon, A. (2009). Functional characterization of a masquerade-like serine proteinase homologue from the black tiger shrimp Penaeus monodon. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 153: 236-243.
  52. Pongsomboon, S., Udomlertpreecha, S., Amparyup, P., Wuthisuthimethavee, S., Tassanakajon, A. (2009). Gene expression and activity of carbonic anhydrase in salinity stressed Penaeus monodon. Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 152: 225-233. .
  53. Ponprateep, S., Somboonwiwat, K., Tassanakajon, A. (2009). Recombinant anti-lipopolysaccharide factor isoform 3 and the prevention of vibriosis in the black tiger shrimp, Penaeus monodon. Aquaculture. 289: 219-224.
  54. Tharntada, S., Ponprateep, S., Somboonwiwat, K., Liu, H., Söderhäll, I., Söderhäll, K., Tassanakajon, A. (2009). Role of anti-lipopolysaccharide factor from the black tiger shrimp, Penaeus monodon, in protection from white spot syndrome virus infection. Journal of General Virology. 90: 1491-1498.
  55. Vatanavicharn, T., Supungul, P., Puanglarp, N., Yingvilasprasert, W., Tassanakajon, A. (2009). Genomic structure, expression pattern and functional characterization of crustinPm5, a unique isoform of crustin from Penaeus monodon. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 153: 244-252.
  56. Visetnan, S., Donpudsa, S., Supungul, P., Tassanakajon, A., Rimphanitchayakit, V. (2009). Kazal-type serine proteinase inhibitors from the black tiger shrimp Penaeus monodon and the inhibitory activities of SPIPm4 and 5. Fish and Shellfish Immunology. 27: 266-274.
  57. Yang, Y., Boze, H., Chemardin, P., Padilla, A., Moulin, G., Tassanakajon, A., Pugnière, M., Roquet, F., Destoumieux- Garzón, D., Gueguen, Y., Bachère, E., Aumelas, A. (2009). NMR structure of rALF-Pm3, an anti-lipopolysaccharide factor from shrimp: Model of the possible lipid A-binding site. Biopolymers. 91: 207-220.
  58. Fagutao, F.F., Yasuike, M., Santos, M.D., Ruangpan, L., Sangrunggruang, K., Tassanakajon, A., Takahashi, Y., Ueno, R., Kondo, H., Hirono, I., Aoki, T. (2009). Differential gene expression in black tiger shrimp, Penaeus monodon, following administration of oxytetracycline and oxolinic acid. Developmental and Comparative Immunology. 33: 1088-1092.
  59. Imjongjirak, C., Amparyup, P., Tassanakajon, A., Sittipraneed, S. (2009). Molecular cloning and characterization of crustin from mud crab Scylla paramamosain. Molecular Biology Reports. 36: 841-850.
  60. Amparyup, P., Kondo, H., Hirono, I., Aoki, T., Tassanakajon, A. (2008). Molecular cloning, genomic organization and recombinant expression of a crustin-like antimicrobial peptide from black tiger shrimp Penaeus monodon. Molecular Immunology. 45: 1085-1093.
  61. Supungul, P., Tang, S., Maneeruttanarungroj, C., Rimphanitchayakit, V., Hirono, I., Aoki, T., Tassanakajon, A. (2008). Cloning, expression and antimicrobial activity of crustinPm1, a major isoform of crustin, from the black tiger shrimp Penaeus monodon. Developmental and Comparative Immunology. 32: 61-70.
  62. Tharntada, S., Somboonwiwat, K., Rimphanitchayakit, V., Tassanakajon, A. (2008). Anti-lipopolysaccharide factors from the black tiger shrimp, Penaeus monodon, are encoded by two genomic loci. Fish and Shellfish Immunology. 24: 46-54.
  63. Amparyup, P., Donpudsa, S., Tassanakajon, A. (2008). Shrimp single WAP domain (SWD)-containing protein exhibits proteinase inhibitory and antimicrobial activities. Developmental and Comparative Immunology. 32: 1497-1509.
  64. Somboonwiwat, K., Bachère, E., Rimphanitchayakit, V., Tassanakajon, A. (2008). Localization of anti-lipopolysaccharide factor (ALFPm3) in tissues of the black tiger shrimp, Penaeus monodon, and characterization of its binding properties. Developmental and Comparative Immunology. 32: 1170-1176.
  65. Pongsomboon, S., Tang, S., Boonda, S., Aoki, T., Hirono, I., Yasuike, M., Tassanakajon, A. (2008). Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus. Journal of Biochemistry and Molecular Biology. 41: 670-677.
  66. Pongsomboon, S., Wongpanya, R., Tang, S., Chalorsrikul, A., Tassanakajon, A. (2008). Abundantly expressed transcripts in the lymphoid organ of the black tiger shrimp, Penaeus monodon, and their implication in immune function. Fish and Shellfish Immunology. 25: 485-493.
  67. You, E.M., Chiu, T.S., Liu, K.F., Tassanakajon, A., Klinbunga, S., Triwitayakorn, K., De La Peña, L.D., Li, Y., Yu, H.T. (2008). Microsatellite and mitochondrial haplotype diversity reveals population differentiation in the tiger shrimp (Penaeus monodon) in the Indo-Pacific region. Animal Genetics. 39: 267-277.
  68. Amparyup, P., Jitvaropas, R., Pulsook, N., Tassanakajon, A. (2007). Molecular cloning, characterization and expression of a masquerade-like serine proteinase homologue from black tiger shrimp Penaeus monodon. Fish and Shellfish Immunology. 22: 535-546.
  69. Wongpanya, R., Aoki, T., Hirono, I., Yasuik, M., Tassanakajon, A. (2007). Analysis of gene expression in haemocytes of shrimp Penaeus monodon challenged with white Spot syndrome virus by cDNA microarray. ScienceAsia. 33: 165-174.
  70. Imjongjirak, C., Amparyup, P., Tassanakajon, A., Sittipraneed, S. (2007). Antilipopolysaccharide factor (ALF) of mud crab Scylla paramamosain: Molecular cloning, genomic organization and the antimicrobial activity of its synthetic LPS binding domain. Molecular Immunology. 44: 3195-3203.
  71. Tassanakajon, A., Klinbunga, S., Paunglarp, N., Rimphanitchayakit, V., Udomkit, A., Jitrapakdee, S., Sritunyalucksana, K., Phongdara, A., Pongsomboon, S., Supungul, P., Tang, S., Kuphanumart, K., Pichyangkura, R., Lursinsap, C. (2006). Penaeus monodon gene discovery project: The generation of an EST collection and establishment of a database. Gene. 384: 104-112.
  72. Gueguen, Y., Garnier, J., Robert, L., Lefranc, M.P., Mougenot, I., De Lorgeril, J., Janech, M., Gross, P.S., Warr, G.W., Cuthbertson, B., Barracco, M.A., Bulet, P., Aumelas, A., Yang, Y., Bo, D., Xiang, J., Tassanakajon, A., Piquemal, D., Bachère, E. (2006). PenBase, the shrimp antimicrobial peptide penaeidin database: Sequence-based classification and recommended nomenclature. Developmental and Comparative Immunology. 30: 283-288.
  73. Maneeruttanarungroj, C., Pongsomboon, S., Wuthisuthimethavee, S., Klinbunga, S., Wilson, K.J., Swan, J., Li, Y., Whan, V., Chu, K.H., Li, C.P., Tong, J., Glenn, K., Rothschild, M., Jerry, D., Tassanakajon, A. (2006). Development of polymorphic expressed sequence tag-derived microsatellites for the extension of the genetic linkage map of the black tiger shrimp (Penaeus monodon). Animal Genetics. 37: 363-368.
  74. Somboonwiwat, K., Supungul, P., Rimphanitchayakit, V., Aoki, T., Hirono, I., Tassanakajon, A. (2006). Differentially expressed genes in hemocytes of Vibrio harveyi-challenged shrimp Penaeus monodon. Journal of Biochemistry and Molecular Biology. 39: 26-36.
  75. Somprasong, N., Rimphanitchayakit, V., Tassanakajon, A. (2006). A five-domain Kazal-type serine proteinase inhibitor from black tiger shrimp Penaeus monodon and its inhibitory activities. Developmental and Comparative Immunology. 30: 998-1008.
  76. Jarasrassamee, B., Supungul, P., Panyim, S., Klinbunga, S., Rimphanichayakit, V., Tassanakajon, A. (2005). Recombinant expression and characterization of five-domain Kazal-type serine proteinase inhibitor of black tiger shrimp (Penaeus monodon). Marine Biotechnology. 7: 46-52.
  77. Somboonwiwat, K., Marcos, M., Tassanakajon, A., Klinbunga, S., Aumelas, A., Romestand, B., Gueguen, Y., Boze, H., Moulin, G., Bachère, E. (2005). Recombinant expression and anti-microbial activity of anti- lipopolysaccharide factor (ALF) from the black tiger shrimp Penaeus monodon. Developmental and Comparative Immunology. 29: 841-851.
  78. Tang, S., Popongviwat, A., Klinbunga, S., Tassanakajon, A., Jarayabhand, P., Menasveta, P. (2005). Genetic heterogeneity of the tropical abalone (Haliotis asinina) revealed by RAPD and microsatellite analyses. Journal of Biochemistry and Molecular Biology. 38: 182-190.
  79. Yazawa, R., Watanabe, K., Koyama, T., Ruangapan, L., Tassanakajon, A., Hirono, I., Aoki, T. (2005). Development of gene transfer technology for black tiger shrimp, Penaeus monodon. Journal of Experimental Zoology Part A: Comparative Experimental Biology. 303: 1104-1109.
  80. Supungul, P., Klinbunga, S., Pichyangkura, R., Hirono, I., Aoki, T., Tassanakajon, A. (2004). Antimicrobial peptides discovered in the black tiger shrimp Penaeus monodon using the EST approach. Diseases of Aquatic Organisms. 61: 123-135.
  81. Leelatanawit, R., Klinbunga, S., Puanglarp, N., Tassanakajon, A., Jarayabhand, P., Hirono, I., Aoki, T., Menasveta, P. (2004). Isolation and characterization of differentially expressed genes in ovaries and testes of the giant tiger shrimp (Penaeus monodon). Marine Biotechnology. 6: S506-S510.
  82. Tang, S., Tassanakajon, A., Klinbunga, S., Jarayabhand, P., Menasveta, P. (2004). Population structure of tropical abalone (Haliotis asinina) in coastal waters of Thailand determined using microsatellite markers. Marine Biotechnology. 6: 604-611.
  83. Amparyup, P., Klinbunga, S., Preechaphol, R., Tassanakajon, A., Hirono, I., Aoki, T. and Jarayabhand, P. (2004). Expressed sequence tag analysis of ovaries and testes from tropical abalone (Haliotis asinina). Marine Biotechnology 6: S365-S370.
  84. Klinbunga, S., Amparyup, P., Leelatanawit, R., Tassanakajon, A., Hirono, I., Aoki, T., Jarayabhand, P., Menasveta, P. (2004). Species identification of the tropical abalone (Haliotis asinina, Haliotis ovina, and Haliotis varia) in Thailand using RAPD and SCAR markers. Journal of Biochemistry and Molecular Biology. 37: 213-222.
  85. Klinbunga, S., Amparyup, P., Thamrungtanakit, S., Tassanakajon, A., Hirono, I., Aoki, T., Jarayabhand, P. and Menasveta, P. (2004). Population genetics and special-specific markers of tropical abalone (Haliotis asinina) in Thailand. Marine Biotechnology 6: S360-S364.
  86. Tassanakajon, A. (2003). Genetic diversity and population structure of the black tiger shrimp, Penaeus monodon, in Thailand revealed by microsatellite markers. Journal of Research Methodology. 16: 359-380.   
  87. Klinbunga, S., Khamnamtong, N., Tassanakajon, A., Puanglarp, N., Jarayabhand, P., Yoosukh, W. (2003). Molecular genetic identification tools for three commercially cultured oysters (Crassostrea belcheri, Crassostrea iredalei, and Saccostrea cucullata) in Thailand. Marine Biotechnology. 5: 27-36.
  88. Klinbunga, S., Pripue, P., Khamnamtong, N., Puanglarp, N., Tassanakajon, A., Jarayabhand, P., Hirono, I., Aoki, T., Menasveta, P. (2003). Genetic Diversity and Molecular Markers of the Tropical Abalone (Haliotis asinina) in Thailand. Marine Biotechnology. 5: 505-517.
  89. Supungul, P., Klinbunga, S., Pichyangkura, R., Jitrapakdee, S., Hirono, I., Aoki, T., Tassanakajon, A. (2002). Identification of immune-related genes in hemocytes of black tiger shrimp (Penaeus monodon). Marine Biotechnology. 4: 487-494.
  90. Supangul, P., Klinbunga, S., Pichyangkura, R., Hirono, I., Aoki, T. and Tassanakajon, A. (2002).  Immune-related genes in haemocytes of the black tiger shrimp, Penaeus monodon, infected with Vibrio harveyi, Fisheries Science. (suppl II) 68: 1083-1086.
  91. Wilson, K., Li, Y., Whan, V., Lehnert, S., Byrne, K., Moore, S., Pongsomboon, S., Tassanakajon, A., Rosenberg, G., Ballment, E., Fayazi, Z., Swan, J., Kenway, M., Benzie, J. (2002). Genetic mapping of the black tiger shrimp Penaeus monodon with amplified fragment length polymorphism. Aquaculture. 204: 297-309.
  92. Klinbunga, S., Amparyup, P., Khamnamtong, N., Tassanakajon, A., Jarayabhand, P., Yoosukh, W. (2002). Molecular genetic markers for taxonomy of oysters in Thailand. Fisheries Science. (suppl II) 68: 1087-1090.
  93. Jarayabhand, P., Pripue, P., Khamnamtong, N., Klinbunga, S., Tassanakajon, A. (2002). Identification of species-diagnostic markers of abalone inThailand using PCR-RFLP of 16S rDNA. Fisheries Science. (suppl II) 68: 1091-1094.
  94. Kiatpathomchai, W., Boonsaeng, V., Tassanakajon, A., Wongteerasupaya, C., Jitrapakdee, S., Panyim, S. (2001). A non-stop, single-tube, semi-nested pcr technique for grading the severity of white spot syndrome virus infections in Penaeus monodon. Diseases of Aquatic Organisms. 47: 235-239.
  95. Klinbunga, S., Ampayup, P., Tassanakajon, A., Jarayabhand, P., Yoosukh, W. (2001). Genetic diversity and molecular markers of cupped oysters (Genera Crassostrea, Saccostrea, and Striostrea) in Thailand revealed by randomly amplified polymorphic DNA analysis. Marine Biotechnology. 3: 133-144.
  96. Klinbunga, S., Siludjai, D., Wudthijinda, W., Tassanakajon, A., Jarayabhand, P., Menasveta, P. (2001). Genetic heterogeneity of the giant tiger shrimp (Penaeus monodon) in Thailand revealed by RAPD and mitochondrial DNA RFLP analyses. Marine Biotechnology. 3: 428-438.
  97. Pongsoomboon, S., Whan, V., Moore, S., and Tassanakajon, A. (2000) Characterization of tri- and tetranucleotide microsatellite in the black tiger prawn, Penaeus monodon. ScienceAsia 26: 1-8.
  98. Supungul, P., Sootanan, P., Klinbunga, S., Kamonrat, W., Jarayabhand, P., Tassanakajon, A. (2000). Microsatellite polymorphism and the population structure of the black tiger shrimp (Penaeus monodon) in Thailand. Marine Biotechnology. 2: 339-347.
  99. Klinbunga, S., Ampayup, P., Tassanakajon, A., Jarayabhand, P., Yoosukh, W. (2000). Development of species-specific markers of the tropical oyster (Crassostrea belcheri) in Thailand. Marine Biotechnology. 2: 476-484.
  100. Klinbunga, S., Penman, D.J., McAndrew, B.J., Tassanakajon, A. (1999). Mitochondrial DNA diversity in three populations of the giant tiger shrimp Penaeus monodon. Marine Biotechnology. 1: 113-121.
  101. Tassanakajon, A., Pongsomboon, S., Jarayabhand, P., Klinbunga, S., Boonsaeng, V. (1998). Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. Journal of Marine Biotechnology. 6: 249-254.
  102. Tassanakajon, A., Tiptawonnukul, A., Supungul, P., Rimphanitchayakit, V., Cook, D., Jarayabhand, P., Klinbunga, S., Boonsaeng, V. (1998). Isolation and characterization of microsatellite markers in the black tiger prawn Penaeus monodon. Molecular Marine Biology and Biotechnology. 7: 55-61.
  103. Kanchanaphum, P., Wongteerasupaya, C., Sitidilokratana, N., Boonsaeng, V., Panyim, S., Tassanakajon, A., Withyachumnarnkul, B., Flegel, T.W. (1998). Experimental transmission of white spot syndrome virus (WSSV) from crabs to shrimp Penaeus monodon. Diseases of Aquatic Organisms. 34: 1-7.
  104. Tassanakajon, A., Pongsomboon, S., Rimphanitchayakit, V., Jarayabhand, P., Boonsaeng, V. (1997). Random amplified polymorphic DNA (RAPD) markers for determination of genetic variation in wild populations of the black tiger prawn (Penaeus monodon) in Thailand. Molecular Marine Biology and Biotechnology. 6: 110-115.
  105. Wongteerasupaya, C., Tongchuea, W., Boonsaeng, V., Panyim, S., Tassanakajon, A., Withyachumnarnkul, B., Flegel, T.W. (1997). Detection of yellow-head virus (YHV) of Penaeus monodon by RT-PCR amplification. Diseases of Aquatic Organisms. 31: 181-186.
  106. Auditho, M., Tassanakajon, A., Boonsaeng, V., Tpiankijagum, S., Panyim, S. (1995). Simple nonradioactive DNA hybridization method for identification of sibling species of Anopheles dirus (Diptera: Culicidae) complex. Journal of Medical Entomology. 32: 107-111.
  107. Wongteerasupaya, C., Vickers, J.E., Sriurairatana, S., Nash, G.L., Akarajamorn, A., Boonsaeng, V., Panyim, S., Tassanakajon, A., Withyachumnarnkul, B., Flegel, T.W. (1995). A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. Diseases of Aquatic Organisms. 21: 69-77.
  108. Wongteerasupaya, C., Sriurairatana, S., Vickers, J.E., Akrajamorn, A., Boonsaeng, V., Panyim, S., Tassanakajon, A., Withyachumnarnkul, B., Flegel, T.W. (1995). Yellow-head virus of Penaeus monodon is an RNA virus. Diseases of Aquatic Organisms. 22: 45-50.
  109. Jitrapakdee, S., Tassanakajon, A., Boonsaeng, V., Piankijagum, S., Panyim, S. (1995). A simple, rapid and sensitive detection of salmonella in food by polymerase chain reaction. Molecular and Cellular Probes. 9: 375-382.
  110. Boonsaeng, V., Tassanakajon, A.,  Wongteerasapaya, C., Pumichoti, P.,  and Panyim, S. (1994). Simple non-radioisotopic DNA fingerprinting with digoxigenin labelled M13 phage using chromogenic and chemiluminescent detection systems. J. Sci. Soc.Thailand., 19 : 47-55.
  111. Tassanakajon, A., Boonsaeng, V., Wilairat, P., Panyim, S. (1993). Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes. Transactions of the Royal Society of Tropical Medicine and Hygiene. 87: 273-275.
  112. Tirasophon, W., Tassanakajon, A., Boonsaeng, V., Panyim, S., Wilairat, P. (1993). Sensitive detection of Plasmodium falciparum in blood and mosquito by DNA amplification. Parassitologia. 35 Suppl: 117-120.
  113. Tassanakajon, A., Wongteerasapaya, C., Pumichoti, P., Boonsaeng, V., Panyim, S. (1991). Improved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA. Molecular and Cellular Probes. 5: 111-115.
  114. Tassanakajon, A., Hill, W.E. (1988). A unique structure at cytidine 1400 of 16S ribosomal RNA of Escherichia coli. Advances in Biochemistry and Biotechnology in Asia and Oceania.
  115. Hill, W.E., Tassanakajon, A. (1987). Probing ribosome structure using short oligodeoxyribonucleotides: the question of resolution. Biochimie. 69: 1071-1080.
 
Academic Staff
 

Anchalee   TASSANAKAJON
Alisa   VANGNAI
Aran   INCHAROENSAKDI
Boonyarin   JARASRASSAMEE
Kanoktip   PACKDIBAMRUNG
Kuakarun   KRUSONG
Kunlaya   SOMBOONWIWAT
Lerson   TANASUGARN
Manchumas   PROUSOONTORN
Napa   SIWARUNGSON
Nuchanat   WUTIPRADITKUL
Pakorn   WINAYANUWATTIKUN
Piamsook   PONGSAWASDI
Rath   PICHYANGKURA
Saowarath   JANTARO
Siriporn   SITTHIPRANEED
Somporn   KAMOLSIRIPICHAIPORN
Suchart   CHANAMA
Supaart   SIRIKANTARAMAS
Surasak CHUNSRIVIROT
Tanakarn   MONSHUPANEE
Thanyada   ROONGROJMONGKOL
Teerapong   BUABOOCHA
Tikamporn   YONGVANICH
Tipaporn   LIMPASENI
Vichien   RIMPHANITCHAYAKIT

 
Department of Biochemistry, fl. 5, Klum Watcharobol Bld. Faculty of Science, Chulalongkorn University, Payathai Road, Patumwan, Bangkok 10330 Thailand Tel: 662-218-5416-7 Fax: 662-218-5418